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文献和实验Two-hybrid analysis of genetic
a protein that both activates transcription and is toxic to yeast,Drosophila Cyclin E (Finley,Zavitz,Thomas,Richardson,Zipursky,and Brent,in prep),is discussed in Section 7. Figure 1.Mating assay for interactions between a prey and 96 baits Figure
Studies of the Ubiquitin Proteasome System
) Dialysis buffer (see recipe ; include DTT) Glycerol Refrigerated centrifuge 1.5‐ml screw‐cap microcentrifuge tubes End‐over‐end rotator 8000‐MWCO dialysis
Two-hybrid analysis of genetic regulatory networks
mating together with a large panel of bait strains to characterize a protein that both activates transcription and is toxic to yeast, Drosophila Cyclin E (Finley, Zavitz, Thomas, Richardson, Zipursky, and Brent, in prep), is discussed in Section
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