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文献和实验under hemocytometer coverslips.] 4) Stain cells for CD3 expression and evaluate by flow cytometry to calculate the number of CD3 T cells in the bag. 5) Determine the total cell volume by weighing the bag. 6) When CD3 T cell
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
1) Resuspend the Dynabeads Mouse T-Activator CD3/CD28 in the vial. 2) Transfer the desired volume of Dynabeads to a tube. 3) Add an equal volume of Buffer, or at least 1 ml, and mix (vortex for 5 seconds
UV Absorbance (280 nm) Protein Determination
. (Protein Determination by UV Absorption - Alastair Aitken and Michale Learmonth - Protein Protocols in CD Rom - Humana Press, 1998) Introduction Quantitation of the amount of protein in a solution is possible in a simple spectrometer
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