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文献和实验Serum Creatine Kinase in Progressive Muscular Dystrophies
Several enzymes originating in muscle show increased activity in the serum of patients affected with different forms of muscular dystrophy (MD). These enzymes include creatine kinase (CK), aldolase, transaminase, and pyruvate kinase (PK
Chromatin Immunoprecipitation (ChIPs) Protocol (Farnham Lab)
that can not be efficiently sonicated. 2. Stop the crosslinking reaction by adding glycine to a final concentration of 0.125 M. Continue to rock or spin at room temp for 5 minutes. 3. For adherent cells, pour off media and rinse plates
Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues(Farnham Lab)
a difference with different types of media. 1X PBS should also work. Add formaledehyde to a final concentration of 1% and rotate tube at room temperature for 15 minutes. We generally use 0.03g of tissue per antibody. The exact amount of tissue
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