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上海希言科学仪器有限公司
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文献和实验was consistent and even over entire column run @1.0ml/5min. Washed with 10 volumes of 0.1M KCl Buffer C (20ml)each. Collected 10-2.0ml fractions of washes = pool 1-3,4-10. All fractions collected were 2.0ml and contained 8mg of insulin as carrier. Eluted
Preparation of Affinity Column
Preparation of Affinity Column SEK 5/3/95 1.Add 222.2μl 1M MOPS,pH 7.5 to 2ml of 10mg of CREBtide (0.1M MOPS pH 7.5 final concentration). 2.Read OD205,OD280 (using 0.1M MOPS buffer as blank) 3.Affigel-10 is stored frozen at 4.Filter 1-2
Preparation of Affinity Column制备亲和层析柱【UCSF】
and vacuum.Do not allow beads to dry. 5.Wash bed with 3x2ml dH2O. 6.Scrape 0.5-1ml into 15ml microfuge tube. 7.Add 2.22ml CREBtide ligand solution to tube. Rock at 4℃ for 4hrs. 8.Transfer slurry to column. 9.Wash 2x500μl dH2O. 10.Collect eluate
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