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文献和实验Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses
found in clinical samples (variations in cellular functions, lineages, and clonotypic breadth) requires approaches for monitoring immune responses with single-cell resolution. In this chapter, we present an engineering approach for integrated single-cell
Sample preparation (analytical gels)
protein solubilization. The SWISS-2DPAGE samples were prepared as followed: Human samples CEC (Colon epithelial cell): Within a maximum of 30 min, a large right or left colon tissue sample (5 x 7 cm) was prepared on ice in the operating room
Analytical Centrifugation: Equilibrium Approach
representation of an analytical ultracentrifuge. The rotor (C ) has holes through it to hold sample containers commonly called cells (D ). Each cell (see inset) consists of a centerpiece (G ) with open‐sided chambers called channels (H ) to hold the liquid
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