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上海希言科学仪器有限公司
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文献和实验Materials Bunsen burner Wire loop Petri plate or broth tube Bacterial culture Microscope slides Procedure 1. Pick up the inoculating loop and hold it pointed down into an open flame until the loop glows red
: Use 4X stacking buffer at 1:8, 10% SDS at 1:5, and b-ME at 70μl/10ml (e.g.10mls strip=1.25mls 4X, 2mls 10% SDS, 70μl b-ME & 6.68mls H2O) Lysis of Cultures Cells General Protocol 1) Wash cells twice with ice-cold PBS. Use 3mls (6cm plate
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
12ul 10xTBE 32ul dyes (0.8% BPB & XLC) heat to 95C 2'', cool on ice, warm to RT prior to loading 4) Running: attach aluminum plate. At 2000v-2500v, short sequence xc 27cm 2hr, medium xc 65cm 4hr, long xc 110cm 7hr or 1200v o/n 5) Fixing
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