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文献和实验Preparation of Genomic DNA from Bacteria- using Phase Lock GelTM
. Transfer 1.5 ml to a micro centrifuge tube and spin 2 min. Decant the supernatant. Repeat with another 1.5 ml of cells. Drain well onto a Kimwipe. 2. Resuspend the pellet in 467 μl TE buffer by repeated pipetting. Add 30 μl of 10% SDS and 3 μl
are precipitated with sodium acetate, and the lysate is cleared first by filtration of precipitate through cheesecloth and then by centrifugation. The DNA-containing supernatant is transferred to a new tube, and the plasmid or cosmid DNA is precipitated
flow-through drain out (sample) 10) Wash column with 2x50 ml of wash buffer (50 mM NaH2PO4, 300 mM NaCl, 20 mM imidazole, pH 8.0) 11) Elute protein in five 5 ml fractions with elution buffer (50 mM NaH2PO4, 300 mM NaCl, 250 mM imidazole, pH
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