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文献和实验Sample preparation (analytical gels)
containing urea (8 M), CHAPS (4% w/v), Tris (40 mM), DTE (65 mM) and a trace of bromophenol blue. Sixty μl (45 μg) of the final diluted lymphoma sample was loaded on the first dimensional separation. PLASMA: An aliquot of 6.25 μl of human plasma was mixed
Analytical Centrifugation: Equilibrium Approach
representation of an analytical ultracentrifuge. The rotor (C ) has holes through it to hold sample containers commonly called cells (D ). Each cell (see inset) consists of a centerpiece (G ) with open‐sided chambers called channels (H ) to hold the liquid
Purification Techniques of Subcellular Compartments for Analytical and Preparative Purposes
As analytical approaches, we propose procedures to label the plasma membrane and the endo-lysosomal compartments by biotinylation and to separate early and late compartments on a Percoll gradient.
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