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文献和实验Marcantonio Lab Protocol Manual
NaOAc 5.2 see "Molecular Biology Solutions" Competent HB-101s Preparation of Competent Cells (HB-101) 1) Inoculate 50 ml of L2 media in a 250 ml flask with a single colony of cells or a frozen stock
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
B5 vitamines (1ml of 1000x stock) (SIGMA; #G-2519) Gamborg''s vitamine powder, to prepare the 1000x stock disolve 11.2g in 100ml water. 5% sucrose adjust to pH 5.7 before autoclaving after autoclaving add: - Benzylamino Purine (BAP
IntroductionA major advance in our knowledge of cells came about with the ability to maintain them in continous culture. Prokaryotes have been cultured for a relatively long time, but eukaryotic cultures were first accomplished in the early 1900's
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