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文献和实验on a Source Q column. Preparing Yeast Whole Cell Extracts Yeast Strain: The strain used for this method is SHY282.In this strain,the chromosomal TFC4 had been disrupted with the HIS3 gene.It was then transformed with a centromeric plasmid (pRS314 vector
Purification of TFIIIC from yeast
on a Source Q column. Preparing Yeast Whole Cell Extracts Yeast Strain: The strain used for this method is SHY282.In this strain,the chromosomal TFC4 had been disrupted with the HIS3 gene.It was then transformed with a centromeric plasmid (pRS314 vector
; average, 150 kb) in Escherichia coli cells. Based on naturally occurring F-factor plasmid found in the bacterium E. coli. Compare cloning vector . Bacteriophage: See phage. Base pair (bp): Two nitrogenous bases (adenine and thymine or guanine
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