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文献和实验Preparation of cytospin from single cell suspension.
Cell number, speed and trivial details affect cytospin . Basic protocol: Prepare a cell suspension of not more than 0.5 x 106 cells / ml of protein-containing medium. Pre-label the slides. Procedure for old type
From In Vitro to In Vivo: Imaging from the Single Cell to the Whole Organism
, or Cell Lines Basic Protocol 1: Cuvette‐Based Spectrofluorometry to Assess Second‐Messenger Signaling in Living Cells Support Protocol 1: Enhanced Calcium Loading Techniques with Fura‐2 and Fluo‐4
of cells for each electroporation). 2. Allow to sit at room temperature for 5 minutes (this step may be omitted). 3. Aliquot the cell/DNA mixture into electroporation cuvettes (0.9 ml per cuvette; the volume is important!). Place the cuvette
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