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文献和实验) or other appropriate secondary antibody Ponceau S Stain 0.1 g Ponceau S 5 ml acetic acid 95 ml ddH2O Western Transfer - traditional tank method while running the gel, place transfer sandwich and sponges in a tray containing Towbin buffer
I and replace with Destaining Solution II. Addition of Kimwipes to one corner of the staining tray will help remove Coomassie blue from the gel without changing the destaining solution. Replace tissues when they are saturated with Coomassie blue.
Staining protocols for placental explant cultures
nitrogen. It is convenient to keep the tissue near the top of the cryotube. Liquid nitrogen-cooled isopentane may also be used for freezing. Tissue may be kept frozen for several weeks. Precoat cleaned glass microscope slides with poly-L-lysine at 100 mg/ml
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