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文献和实验.6. Load 10ul - 25ul (10ul for a 15 tooth mini comb) and then overlay samples with 1 x SDS running buffer. Empty wells should have 10-15ul sample buffer added and be overlayed as with the test samples so the gel runs straight. Flood the upper chamber
.6. Load 10μl- 25μl(10μl for a 15 tooth mini comb) and then overlay samples with 1 x SDS running buffer. Empty wells should have 10-15μl sample buffer added and be overlayed as with the test samples so the gel runs straight. Flood the upper chamber
Purifying Large E. coli Restriction Fragments from Pulsed-Field Gels
molecular weight standard. 7-Fill all empty wells and, if necessary, top off sample wells with molten 1% agar ose. Gel setup 1-Fill gel chamber with 2 l 0.5x TBE. Turn on cooler and set it to 14 °C. Turn on pump, set the pump speed
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