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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
9. GlutaMAX™-I Supplement 10. Basic FGF, Recombinant Human 11. β-Mercaptoethanol, 1000X 12. Penicillin-Streptomycin, Liquid 13. Attachment Factor 14. TrypLE™ Select Cell Dissociation Reagent or 0.05% Trypsin/EDTA
Northern Blotting方法(Howell Lab)
5 ml of scintillation fluid and count. % incorporation = (cpm of washed/cpm of unwashed) x 100. Anything over 10% is usually usable. Using the kit, it's usually 40%. The specific activity (S.A.) of the probe is also important. S
Biotin labeling and hybridization of probe to chromosomes
20 X SSC 2 µg sheared salmon sperm DNA (10 mg/ml) 2 µl probe DNA
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