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文献和实验3% TREVIGEL PROTOCOL for 3 bp resolution
.) The gel does not flow easily so you must start at one end of the tray and quickly pour the gel to the other end of the tray. Carefully insert the combs and let gel set up at least 15 min. *Note : Having EtBr present in the gel
like the Biorad model 1000/500 you can do a run like this automatically (without having to be in lab to switch up the Amps in the middle or shut them off at the end). Alternatively, for overnight runs, run the gel at 11 mAmps and set the timer to turn the gel
Alkaline Southern Blotting Procedure
and an additional 1 sec on gel alone. Invert gel, place into .25M HCl for 7 min. Rinse briefly in dH2O. Soak in 0.3M NaOH,0.3M NaCl for 10 min. Set up a transfer tray (9 X 12 inch glass baking dish) by pouring transfer solution in the tray (about 1000 ml
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