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文献和实验v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
-loading pipette tips, then loadequal volumes of each sample into the other lanes (usually4–40 μL containing 2–20 μg of total protein). 4. Connect the tank to a power supply and run the gels at 120 Vfor 20 min at room temperature, then at 180 V
Site-directed hydroxy radical mapping of nucleosome positions in vitro
in the ranges approximately 75-300 bp and 25-200 bp respectively. We use BioRad SequiGen gel equipment with a BioRad 3000 power supply with temperature control set to maintain a constant 50°C gel temperature. Gels are pre-run for 45-60 minutes to bring
apparatus to accommodate 16 × 22–cm glass plates (see protocol 2 ) Power supply capable of running in constant current mode, with two leads
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