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文献和实验B-Cell Development and Pre-B-1 Cell Plasticity In Vitro
have been very useful in unraveling this complex process of B-cell development. Here the protocols for how to grow and to study the differentiation of mouse pre-B-1, as developed in our laboratory, are described. Moreover, the protocols of how to grow and to test
Activation, proliferation and differentiation of na�ve B lymphocytes into memory B cells and plasma cells requires engagement of the B cell receptor (BCR) coupled to T-cell help (1 , 2) . T cells deliver help in cognate fashion
with 5 ml of PBS and add to gradient. Centrifuge at 300 g for 40 minutes, room temperature. Collect the B cells at the interface. Wash 3 times with PBS. Suspend the SRBC-T cell pellet. Centrifuge at 300 d for 10 minutes. Aspirate all of the supe
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