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文献和实验UPDATED DIRECTIONS FOR NEW BRUNSWICK MOBILE PILOT PLANT FERMENTOR
Hahn Lab Check manuals for more detailed information . Please Note: We have been having problems with the seal and bearing assembly at the bottom of the unit which allows the shaft to rotate inside the growth chamber.
Purifying Large E. coli Restriction Fragments from Pulsed-Field Gels
2-Shake gently at room temperature approximately 15 min. 3-Aspirate off the EC lysis solution (be careful not to suck up the agarose plug!). 4-Add 1 ml per well EC lysis solution with lysozyme and RNase. 5-Seal plate inside a heat-sealable bag
.Wear gloves for the following steps7. Cut 2 pieces of large 3MM paper (wicks) and 2 pieces about 2mm smaller than the gel on each edge and one piece of nylon (Hybond N , Amersham) the same size as the gel.Large gel (HFI) Medium gel Mini gel2 (14 x 19cm
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