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文献和实验Sequencing off Cosmid, BAC, PAC, and Fosmid Templates with ABI Big Dye Terminators
Big Dye Protocols and Notes - Cosmid, BAC, BAC, Fosmid Templates Hi all, Over the past two months, we have been testing various reaction conditions for the ABI Big-Dye Terminators with double-stranded DNA templates ranging from pUC
Wholemount in situ hybridisation
). Incubate at 37°C for 15 minutes. Pack a Sephadex G50 column equilibrated with 0.1% SDS, 50mM Tris.Cl (pH 7.5), 0.5mM EDTA (TES) by loading a 1ml syringe barrel (plugged with glass or plastic wool) and spinning @ 1500rpm 90sec. Dilute probe
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