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文献和实验Transformation of E. coli by Electroporation
number of microcentrifuge tubes. Place the required number of Micro-electroporation Chambers on ice. Fill the temperature control compartment of the Chamber Safe with ~250 ml of ice-water slurry and place the Chamber Rack in the Chamber Safe. 2. Thaw
by inverting the bottle a few times, and incubate in an ice-water bath for 30-60 minutes. 6. Clear the lysate of precipitated SDS, proteins, membranes, and chromosomal DNA by pouring through a double-layer of cheesecloth. Transfer the lysate into 250 ml
Medium Per Liter: To 950 ml of deionized H2 O , add: Tryptone 20 g Yeast Extract 5 g NaCl 0.5 g Shake until the solutes have dissolved. Add 10 ml of a 250 mM solution of KCl. (This solution is made by dissolving
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