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上海希言科学仪器有限公司
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文献和实验Hepes. Harvest cells by putting 20mls of -Ca-Mg PBS, buffered with 0.02M Hepes, and 1mM EDTA on each plate for 10min in the 37 C incubator. After the 10min, collect some of the PBS in a 50ml conical tube before pipeting
(4heads/10ul) 4. Spin at 112000 rpm in an Eppendorf microfuge for 10 min at 4°C. 5. Transfer the supernatant to a new tube and discard the pellet. 6. Boil for 3 min at 99°C. 7. store at 4°C. B. Polyacrylamide gel 1. Resolving gel: 10 ml
the stock uncoupled microsphere suspension according to the instructions described in the Product Information Sheet provided with your microspheres. 2.Transfer 5.0 x 106 of the stock microspheres to a USA Scientific microcentrifuge tube.
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