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文献和实验High-Content Screening: Flow Cytometry Analysis
The HyperCyt� high-throughput (HT) flow cytometry sampling platform uses a peristaltic pump, in combination with an autosampler, and a novel approach to data collection, to circumvent time-delay bottlenecks of conventional
through it with a peristaltic pump. Pump at least 3 column volumes (15 mL) through the column. 4.Sonicate cells for one 20-second cycle. (This should be enough sonication since we used lysozyme above. Over-sonicating disrupts the GST tag and makes it so that the protein
Large Scale Tubulin Preparation
(filtered) 1-1.5L cleaned column housing 2 stirring rods 2 aspirators with large traps Peristaltic pump 10 ml plastic pipets as inlets for peristaltic pump Column Preparation Procedure : 1. Pour 1L 0.5N NaOH into 5 x
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