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文献和实验Plasmid Mini Purification Protocol
buffer will not affect the elution step. - Remove a 50 µl sample of the flowthrough and save for an analytical gel (sample 3) 8. Move the QIAGEN column to a sawed-off 15-ml tube (in the QIAGEN materials box) and press the tube down over a new, labelled
Spin down in Sorvall--5 K, 5 minutes, 4°C. Drain. Resuspend cells in 5 mL HEPES/sorb to wash. Spin down (5 K, 5 minutes, 4°C). Drain. Resuspend in 5 mL HEPES/sorb with 0.5 mM PMSF (add all inhibitors immediately before use). Add 60 µL 1
, 5 minutes, 4°C. Drain. Resuspend cells in 5 mL HEPES/sorb to wash. Spin down (5 K, 5 minutes, 4°C). Drain. Resuspend in 5 mL HEPES/sorb with 0.5 mM PMSF (add all inhibitors immediately before use). Add 60 µL 1 mg/mL
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