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文献和实验Autoradiography using Emulsion-coated Coverslips
1. Cleaning coverslips (use gloves) Add 1 bottle of Chromerge, 1/5 at a time, into 2.5 L bottle of Sulfuric Acid. Invert
microdissected cells in 20 µl proteinase K buffer.Incubate overnight at 37°C.B: Prepare the Glass PlatesTIP: Use Accuwipes for cleaning purposes, as they will not leave lint behind and are non-abrasive.Clean glass plates twice with glass cleaner.Repeat using 95
Immunostaining of Paraffin Sections
in several changes of cool PBS. Infiltrate and embed in paraffin (use automatic processor). Embed in flat end of capped Beem capsules 2) Prepare polylysine-coated slides by immersing clean slides, via a stainless steel slide rack
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