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上海希言科学仪器有限公司
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文献和实验Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
°C for X min(1min/kb) Final extension 72°C for 7 min Final soak 4-10°C 6) For a simplified hot start, proceed as described in step 7. Otherwise, place the PCR tubes in the thermal cycler and start the cycling
6) For a simplified hot start, proceed as described in step 7. Otherwise, place the PCR tubes in the thermal cycler and start the cycling program. 7) Simplified hot start: Start the PCR program. Once the thermal cycler has reached 94°C
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