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文献和实验and forcibly into the oligo dT cellulose. Repeat 1X into a second tube.10. Add 65 l 3M NaAcetate to all samples. Fill the remainder of the tube with room temperature isopropanol （~700 l）. Mix well by inverting several times.11. The sample can now sit at .20 !C
定量RT-PCR （Quantitative RT-PCR）
reflects the input ratio between these two templates. As the amount of input control templates is known, the amount of wild type templates in the RNA sample can be easily determined. The system described below has been developed
at room temperature for 3 minutes and then centrifuge at 14,000 RPM for 1 minute. Try to remove as much buffer as possible without aspirating the pelleted complex. Efficient washing is critical to reduced background. 5.Include the input/starting sample
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