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文献和实验Hints and precautions for the care, feeding and breeding of Neurospora
months later when rehydrated and heatshocked. Intact linear asci may be extruded singly after perithecia that have been dried in this way are placed in liquid. Crosses made in 10 X 75 mm culture tubes often dry down before the perithecia have emptied
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
2. Table top centrifuge capable at least 13,000 x g 3. 1.5 or 2 ml Nuclease-Free centrifuge tubes 4. 0.45 μm filter unit 实验步骤 Before starting: 1. Fresh or flash-frozen cultured cells can be used
upon the dual pulldown to incorporate a third pulldown which is an iteration of the ChIP and is a pulldown for H3K27me3+ (Figure 1b). The third assay described here is the biotin-RNA pulldown of a low-copy RNA that spans the siRNA targeted promoter region
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