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文献和实验Linearization and Purification of the Shuttle Plasmid
1.0 Purpose 1.1 This protocol may be used for the linearization and purification of the shuttle plasmid. 2.0 Materials 2.1 Equipment DNA Gel Electrophoresis Apparatus Heating block
Genetic Analysis of Cytomegalovirus by Shuttle Mutagenesis
) has a genome of 230 kb that encodes more than 170 genes, many of which have unknown functions. Many techniques for the genetic analysis of a herpesvirus have been developed over the past two decades. One such procedure involves the use of a shuttle mutagenesis
BAC Modification Using a RecA Expressing Shuttle Vector System
Bacterial artificial chromosomes (BAC) are F-factor plasmid based vectors that are maintained as 1 or 2 copy plasmids in their bacterial host strain. BACs have the capacity for maintaining large, often more than 300 kb fragments of mammalian DNA
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