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上海希言科学仪器有限公司
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文献和实验mixture containing: 1 m L 10x transcription buffer, 0.5 m L DIG labeling mix, 0.4 m L RNA polymerase (20 U/m L), 0.125 m L RNAguard (40 U/m L), and 3 m L RNAse-free water. Plates are then sealed with adhesive foil and incubated for 2-4 h at 37°C. 3.
to microfuge tube, vortex, and ice for another 10min.) 6) Spin at 14000rpm (~16000xg) for 10min at 4OC. 7) Transfer supernatant to new, pre-chilled microfuge tube. 8) Use 5-10 μl for protein assay, or mix 20 μl with 20 μl 2X sample buffer (or 30 μl
Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC
(see below) Flow Cells : Flow cells can be constructed in many different ways. The most common way is to place two strips of double-stick tape on a glass slide ~7-10 mm apart and cover with a 18x18 or 22x22 mm coverslip. This results in a ~12-15 µl flow cell
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