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上海希言科学仪器有限公司
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文献和实验RNA Purification Protocol for 1-2 x 107 cultured cells
设备 1. Microcentrifuge capable of 3,000 x g 2. 15 ml centrifuge tube 3. Ice bath 4. Ground pestle 实验步骤 1. Remove the media and collect cells. For cells grown in suspension, pellet the cell
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
for 30 seconds. 11. Centrifuge at maximum speed ($13,000 x g) at 4°C for 3 minutes. Aspirate the supernatant and air dry the pellet by inverting the tube on a absorbent paper for 5-10 minutes. 12. Add 50-100 μl of nuclease free water
Hints and precautions for the care, feeding and breeding of Neurospora
, above 30°C. We routinely make crosses on SC slants in 18 X 150 mm tubes, using foam or cotton plugs. Push-on metal or plastic caps should be avoided with cross tubes because they result in more rapid water loss and are more prone to contamination
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