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- 供应商:
上海希言科学仪器有限公司
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文献和实验独有的病毒物种数目最多为249(图2b)。选取丰度top20的病毒物种进行热图展示,发现高丰度的病毒主要包括Siphoviridae phages(Lactococcus phage 1706和Enterobacter phage phiEap-2); Myoviridae phages(Vibrio phage VH7D 和Shewanella sp. phage 3/49); Podoviridae phages(Acinetobacter phage Presley和Cronobacter
Preparation of nucleic acid probes
SP6 promoter sequence immediately adjacent to a multiple cloning site (see Figure 5.4 ). The SP6 RNA polymerase can then be used to initiate transcription from a specific start point in the SP6 promoter sequence, transcribing through any DNA sequence
that, because of its short spacer arm (about 2 A), generates reversible covalent links, mainly between proteins and DNA. Subsequently, chromatin is fragmented by sonication and DNA protein complexes are selectively immunoprecipitated with antibodies raised against proteins
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