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文献和实验The Ted Pella Microwave Oven Model 3450, has been used in this electron microscopy (EM) laboratory for the past 3 yr. In that time, over 750 specimens have been processed in the oven. It has reduced turnaround time from as long as 70 h
Two dimensional peptide mapping
from the protease adsorbing onto the filter. 3. Aspirate the liquid. Wash the membrane extensively with H2O (5 X 1ml). Then wash with freshly-made 0.05 M NH4HCO3 once or twice. 4. Digestion: Add 150 -200 microliter fresh 0.05 M NH4HCO3
and neutralizing solution. Tray with neutralizing solution. Whatman #1 filter paper to layer between filters. 80℃ vacuum drying oven (preheated 1 hour). Hybridization buffer (sterile 5x SSPE; 10x filter sterilized Denhardt's solution; sterile 0.1% SDS; use
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