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文献和实验Sectioning and Mounting Protocol
Sectioning and Mounting Protocol Technovit Glycol Methacrylate: Technical Information and Help From Heraeus Kulzer. Sectioning Sectioning is best done with a rotary or sledge microtome such as the JB-4 Microtome. The features
Bromodeoxyuridine Staining of Mammary Tissue
8.5. 8. Rinse 2 x 3 min in PBS. 7. Treat with 0.2% Triton X-100 for 30 minutes at room temperature. 8. Rinse 3 x 5 min in PBS. 9. Block sections 1-2 hours at room temperature with PGB Superblock (see below) in humid chamber. 10. Remove Superblock
Sectioning and Mounting Protocol
. Retraction is needed to keep the hard sample from brushing against the knife edge on the return stroke, damaging the edge and block face. Motorized sample movement is desirable to get more reproducible cutting speed and force. The best sections
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