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文献和实验Tight junctions (TJs) are intercellular structures in epithelial and endothelial cells, primarily playing �critical roles in cell–cell adhesion. Among their molecular components, claudins are the main constituents as integral membrane
Alkaline Southern Blotting Procedure
the two 3mm sheets cut to size of gel in transfer solution. Place onto membrane. Squeeze out air bubbles. Seal around the edges of the blot by laying down strips of plastic. Place blotting material on top, weight it down with a glass plate and a tray
Flow Cell Assays with Microtubules: Motility/Dynamics in Fluorescence and VE-DIC
GTP + 0.5 mg/ml HSA 3. Flow in at least 3-4 chamber volumes of tubulin mix (i.e. tubulin in BRB80 + 1 mM DTT + 1 mM GTP + 0.5 mg/ml HSA), seal edges with Valap (1:1:1 mix of vaseline:lanolin:paraffin) and begin observation. To avoid surface
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