相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 供应商:
上海希言科学仪器有限公司
- 现货状态:
详询
- 保修期:
详询
- 规格:
详询
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验ELECTROPHORESIS OF DNA IN POLYACRYLAMIDE GELS
Pre-run gels for 30 minutes at 80V. Rinse the wells with running buffer. Load and electrophorese samples at 80V until dyes separate, then boost to up to 150 V. Medium sized polyacrylamide gels can be run overnight at 55-60 V to see all phi-X/Hae III
Transformation of E. coli by Electroporation
the cells to a 17 x 100-mm or 17 x 150-mm polypropylene tube and incubate the cultures with gentle rotation for 1 hour at 37°C. 19. Plate different volumes (up to 200 μl per 90-mm plate) of the electroporated cells onto SOB agar medium containing 20mM
JUST BEFORE USE. We use 0.1M PMSF and Complete mini protease inhibitors (Roche). Cells and Harvesting Harvest SL2 cells (see note 1) by spinning down at 200 x g for 7 minutes and wash x 3 in 40mls of ice cold PBS + 5mM Na butyrate (see note
技术资料暂无技术资料 索取技术资料




