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文献和实验Using Cell‐ID 1.4 with R for Microscope‐Based Cytometry
. Bar = 5 µm. Magnification: 60×. (B ) HEK293 cells fixed, trypsinized, and imaged (see and ), before (left) and after (right) Cell‐ID has located them in the image. Bar = 15 µm. Magnification: 20×. View
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
pellet. H. Pre-warm 6-well plates with 2 ml of complete medium at 37 °C. I. Add 2 μg of Target ID Library (not to exceed 10 μl) per 0.5 ml tube for each transfection. J. Resuspend cells in the 15
E-Z 96 X-press Plasmid Vacuum Protocol
selective antibiotic. Incubate 14-16 hours at 37℃ 600 with vigorous shaking until an OD of 2.0-4.0 is achieved. 2. Pellet 1-2 mL bacterial culture in a 2.0 ml 96-well deep-well plate by centrifugation at 3000 x g for 15 min at room temperature.
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