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文献和实验Transformation of E. coli by Electroporation
ml of SOB (without magnesium) in a 1 liter flask. Grow for 2 to 3 hours with vigorous aeration at 37EC until the cells reach an OD550 = 0.8. 3. Harvest cells by centrifugation at 5000 RPM (2,600 g) in a GSA rotor for 10 min. (Make sure you use
Modified Eberwine (antisense) RNA Amplification Protocol
not to spill. Mix by pipeting 5-10X, then transfer slurry to Phase lock gel tubes. Spin 5 minutes maximum speed (15k x g) at room temperature. Transfer aqueous phase to RNase free 1.5 ml eppendorf tube. Add 70 uL 7.5M ammonium acetate (in DEPC water
Purifying Large E. coli Restriction Fragments from Pulsed-Field Gels
, then treated with deter gents and enzymes which remove the cell wall, proteins and other cellular material. The purified chromosomal DNA remains embed allowfullscreen='true'ded in the agarose plug. Preparation of cell plugs 1-Grow an overnight culture of E
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