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文献和实验100°C Heater block 32P-labled probe use 10 - 20 ng DNA/filter, or about 10 million CPM/filter for Southern's Nylon filter DNA previously blotted onto filter (e
UPDATED DIRECTIONS FOR NEW BRUNSWICK MOBILE PILOT PLANT FERMENTOR
2. With fermentor vessel empty, remove pH probe and calibrate if desired (The probe may not have to be recalibrated very often). To calibrate, immerse the electrode in pH 7 reference buffer, turn the selector switch to pH and rotate the mode switch to zero
Wholemount in situ hybridisation
Wholemount in situ hybridisation Based on Wilkinson protocol, modified by Murray Hargrave (m.hargrave@cmcb.uq.edu.au), Koopman lab A. SYNTHESIS OF PROBE 1 Mix in the following order at room temperature:
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