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文献和实验b-Gal Staining of Murine Embryos
phosphate buffer, 400ml of 1M MgCl2 2ml of 0.5M EGTA X-gal : 40 mg/ml in diemthylformamide, covered by foil and store at -20C. 10% formalin (20L) 95.36g KH2PO4 192g K2HPO4 200 ml 40% formadhyde the pH should be 7.4 Fixtive using fix buffer
Whole Mount X-gal Histochemistry of Transgenic Animal Tissues
quality cedarwood oil and rock gently, 1-4 hours. Replace cedarwood oil once and rock gently overnight. Notes Originally from Bob Hammer; I haven't tried this. The X-gal stain is apparently stable in cedarwood oil.
Analysis of Shark Liver Oil by Thin-Layer and Supercritical Fluid Chromatography
The liver oils of certain shark species contains squalene, 2, 6, 20, 15, 19, 23-hexamethyltetracosahexane, at high levels. Squalene is used in the pharmaceutical, rubber, and surfactants industries (1 ). Squalene is easily hydrogenated
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