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文献和实验Lipoplex Formation Using Liposomes Prepared by Ethanol Injection
than the dry-film. The most efficient DC–Chol/DOPE liposome for gene transfer was molar ratio (1:2) and prepared by a modified ethanol injection method. The enhanced transfection is related to an increase in the release of DNA in the cytoplasm by the large
This chapter presents a detailed protocol for the generation of mature, monocyte-derived dendritic cells (DC) that are loaded or“pulsed” with tumor-associated peptide antigens for use as patient-specific immunotherapy. The protocol can easily
Delivery of Tumor Antigens to Dendritic Cells Using Biodegradable Microspheres
Poly(D,L-lactide-co-glycolide) (PLGA) polymers have been used for the production of biodegradable medical sutures and for controlled drug release for decades. Useful characteristics such as in vivo biodegradability, an adjustable release
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