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上海希言科学仪器有限公司
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文献和实验DNA Recovery With Low Melt Agarose
to visualize fragment when curring as this reduces nicking. Don't use UV box in the darkroom! 6.Melt gel slice at 65℃ 5min. 7.Determine volume, place back at 65℃. 8.Add 2.5 vol. of 20mM Tris pH8, 1mM EDTA, mix by pipetting, place at 65℃ 2min. [(10mL)(20mM)=x
Planar Lipid Bilayer Experiment平面脂双层实验
) to ensure there is no contamination in chamber.11、Add protein of interest. Usually 5ul of a 1:1000 dilution of the original sample is a good place to start. Dilute the protein in 0.1% Triton X-100 (10ul in 10ml total). If using diphytanoylphosphatidyl
on one for 5min and resuspend at a concentration of 1 x 106 cells/ml in media containing 10% DMSO. Aliquot 1ml into cyrotubes and freeze in the -70oC freezer in a Mr Frosty overnight. Once frozen transfer the vials to box D2 in the -70 freezer.
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