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文献和实验Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content
Cell-Surface Staining 1. Place 1 x 106 PBS-washed cells into a tube, add 100 µL of antibody staining solution, and mix well. In addition, prepare cells to be stained with isotype-matched control antibodies, as well as cells
-HCL PH 6.6, and that is better) Buffer PE 10 mM Tris-HCl pH 7.5 80% ethanol Buffer QBT equilibration buffer 750 mM NaCl 50 mM MOPS pH 7.0 15% isopropanol 0.15% triton X-100 Buffer QC wash buffer
the beadbound cells with a magnet. Discard the bead-bound cells and use the remaining untouched, enriched cell population for further flow sorting into the DC subpopulation(s) of interest 实验试剂 Magnet: (Dynal® MPC™), MPC-L for 1–5 ml
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