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文献和实验(the melting temperature of paraffin). The blocks are transferred to pure paraffin in the oven for 1 hour and then into a second pot of melted paraffin for an additional 2--3 hours. During this time the tissue block is completely infiltrated with melted
are transferred to pure paraffin in the oven for 1 hour and then into a second pot of melted paraffin for an additional 2--3 hours. During this time the tissue block is completely infiltrated with melted paraffin. Subsequent to infiltration, the tissue
in the RC5-B using the SS-34 rotor. Gently decant the supernatant, add 80% ethanol, centrifuge as before, decant, and dry the DNA pellet in a vacuum oven. 15a. Resuspend the DNA in 10:0.1 TE buffer. For diatomaceous earth-based purification:
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