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文献和实验Steady State ATPase Assays Coupled Enzyme System
Tubulin (>5 mg/mL) 100 mM Mg・GTP 4 mM Taxol in DMSO PM = 100 mM PIPES pH 6.8 2 mM EGTA 1 mM Mg2 SO4
Ex vivo enumeration of the absolute frequencies of antigen-specific T cells is key for evaluating the immunogenicity of T cell-based vaccines. Currently there are three methods that are widely used to quantify cellular immune responses
Toll-Like Receptor-Dependent Immune Complex Activation of B Cells and Dendritic Cells
ICs formed by a mixture of autoantibodies and cell debris released from dying cells in culture. To better understand the nature of the mammalian ligands that can effectively activate TLR7 and TLR9, we have developed a methodology for preparing ICs
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