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文献和实验Conventional polymerase chain reaction (PCR) enables reliable amplification of 3–4 kb of DNA (1 ) while attempts at optimisation has enabled 15.6 kb of λ DNA to be amplified (2 ). The maximum amplifiable length of PCR is limited by the low
The heat-stable DNA polymerase utilized in the polymerase chain reaction (PCR) has been widely used to amplify DNA fragments since its conception by Kerry Mullis in 1985. However, it soon became apparent that there was a constraint
In this chapter, we detail protocols of long polymerase chain reaction (PCR) and long RT-PCR, which we have found to be versatile, sensitive, and straightforward to optimize. We have used these protocols with success on several different
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