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文献和实验Aim For the majority of manipulations using cell cultures, such as transfections, cell fusion techniques, cryopreservation and subculture routines it is necessary to quantify the number of cells prior to use. Using a consistent number
. No. D8537) using a volume equivalent to half the volume of culture medium. Repeat this wash step if the cells are known to adhere strongly. Pipette trypsin/EDTA (Prod. No. T4049) onto the washed cell monolayer using 1ml per 25cm2 of surface area
Subculture of Adherent Cell Lines
the volume of culture medium. Repeat this wash step if the cells are known to adhere strongly. Pipette trypsin/EDTA (Prod. No. T4049 ) onto the washed cell monolayer using 1ml per 25cm2 of surface area. Rotate flask to cover the monolayer with trypsin. Decant
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