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文献和实验Lipid analysis-Week 3: GAS LIQUID CHROMATOGRAPHY
- should read 180 °C, this is the starting oven temperature. Inj A Temp - should read 260 °C, this is the temperature of the injector port (where the sample is injected into and vaporized). Det B Temp - should read 280 °C
Hints and precautions for the care, feeding and breeding of Neurospora
their contents (P. St. Lawrence), and are conveniently stored for future use. 8. Heatshock. For heatshock, a 60°C water bath is preferable to a hot-air oven. (Greater latent heat, better heat transfer, more stable). Allow at least 30 minutes at 60°C to assure
Standard hybridization technique
). You will need: 6 x SSC (0.9M NaCl,90mM sodium citrate,pH 7) 10% SDS 50mg/ml heparin sulphate (Sigma) Standard hybridisation solution: 6 x SSC,500μg/ml heparin,1% SDS. N.B: A modification used for the hybridisation buffer which can also give extremely good results
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