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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
Wavel Plate, ? Wave Retardation, 400-700nm Newport 10RP52-1 1 Rotation Stage, 1" Aperature Newport
of a pure culture, that is, one in which all organisms are descendants of the same organism. 单细胞挑取法 简易单细胞挑取法 micromanipulator 平板分离法 streak plate method The most common way of separating bacterial Cell s on the agar surface
-mm Nalgene filter. Divide into aliquots and store at -20ºC. Pick a single bacterial colony (2-3 mm in diameter) from a plate that has been incubated for 16-20 hours at 37ºC. Transfer the colony into 25 ml of LB
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