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上海希言科学仪器有限公司
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文献和实验Hints and precautions for the care, feeding and breeding of Neurospora
, above 30°C. We routinely make crosses on SC slants in 18 X 150 mm tubes, using foam or cotton plugs. Push-on metal or plastic caps should be avoided with cross tubes because they result in more rapid water loss and are more prone to contamination
In vitro transcription reaction
1 mM UTP (pH 7) 5 ml (a-32P )-UTP (400-800 Ci/mmol; 10 mCi/ml) {final conc. 4-8 mM} 0.2 ml T7 or T3 or SP6 RNA polymerase (50 U/ml, Stratagen) 25 ml, mix, spin incubate at 37 oC for 30 min. Start: End: Add 1ml DNase 1(free from Rnase -10 U/ml; BMB
In vitro transcription reaction
7) 1 ml 10 mM GTP (pH 7) 1 ml 1 mM UTP (pH 7) 5 ml (a-32P )-UTP (400-800 Ci/mmol; 10 mCi/ml) {final conc. 4-8 mM} 0.2 ml T7 or T3 or SP6 RNA polymerase (50 U/ml, Stratagen) 25 ml, mix, spin incubate at 37 oC for 30 min. Start: End: Add 1ml DNase
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