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上海希言科学仪器有限公司
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
background. 4. Incubate tissue in X-Phos/NBT Detection Buffer for 15' at room temperature. XPhos/NBT Detection Buffer (Buffer 3 as described for Genius kit by Boehringer-Mannheim) 100 mM Tris-HCl, pH 9.5 100 mM NaCl 50 mM MgCl
Plant DNA methylation analysis by bisulfite genomic sequencing
Transfer column with resin to 1.5ml reaction tube, add 50µl bidist. water preheated to 64°C for DNA elution; Incubate 1 minute at room temperature; Centrifuge Eppendorf 5414 2 minutes; To DNA solution (~50µl), add 40µl bidist. water
Western Blot with Platelet Protein
according to the manufacturer's instructions. Visualize the chemoluminiscence with the X-Ray film. SOLUTIONS complete lysis buffer = 50 mM Tris HCl, 150 mM NaCl, 1mM EDTA, 1% triton X-100, 10% glycerol, 1 mM NaF, 1 µg/ml pepstatin A, 1 µg/ml aprotinin, 1 µ
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